Court papers filed last week in the US Virgin Islands valued Epstein's estate at more than $577 million (£475m) but listed no details of beneficiaries, the Associated Press reported.
The will, details of which were first reported by the New York Post, directs Epstein's assets to be put into trust.
Epstein died while awaiting trial on s.e.x trafficking and conspiracy charges.
The New York medical examiner found that the 66-year-old, whose body was discovered on 10 August, died of "suicide by hanging".
Epstein pleaded not guilty to the charges against him and was being held without bail. He faced up to 45 years in prison if convicted.
The former financier put all of his holdings into a trust called The 1953 Trust, according to a copy of the will published by the New York Post. He signed the document on 8 August.
No details of any beneficiaries are included in the document, which lists assets including more than $56m in cash, more than $14m in fixed income investments and more than $18m in "aviation assets, automobiles and boats".
Epstein's collection of fine arts, antiques and other valuables is yet to be appraised, the document says.
Some of Epstein's alleged victims have said they will go after his assets for damages following his death.
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